TECHNICAL ISO/TR REPORT 19693 First edition 2018-02 Surface chemical analysis Characterization of functional glass substrates for biosensing applications Analyse chimique des surfaces - Caracterisation de substrats de verre fonctionnels pour les applications de biodétection Reference number IS0/TR 19693:2018(E) so @ IS0 2018 IS0/TR 19693:2018(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2018 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either IsO at the address below or Iso's member body in the country of the requester. ISO copyright office CP 40i: Ch. de Blandonnet 8 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org Published in Switzerland ii @ IS0 2018 - All rights reserved IS0/TR 19693:2018(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. .1 2 Normative references ..1 3 Terms and definitions 4 Symbols and abbreviated terms .2 5 Characterization of substrates for biosensors by surface chemical analysis .2 5.1 Introduction 2 5.2 Surface chemical analysis (SCA) .3 5.2.1 General .3 5.2.2 X-ray photoelectron spectroscopy (XPS) .3 5.2.3 Time-of-flight secondary ion mass spectrometry (ToF-SIMS) .4 5.2.4 Near-edge X-ray absorption fine structure (NEXAFS) spectroscopy .4 5.2.5 Other methods .5 5.3 Characterization ofmicroarray glass substrates. .6 5.3.1 Glass surface composition .6 5.3.2 Characterization of cleaned and pre-activated glass slides ..7 5.4 Characterization of functional silane films formation on glass substrates ..8 5.5 Stability and shelf-life of aminosilane-based biosensing platforms on glass ..12 5.6 Other types of functionalized glass slides. .17 6 Concluding remarks .21 Annex A (informative) Study of pre-cleaned commercial glass slides. .25 Bibliography .28 @ IS0 2018 - All rights reserved iii